Using 1.5 mm internal diameter columns for optimal compatibility with current liquid chromatographic systems

This article describes the use of a new prototype column hardware made with 1.5 mm internal diameter (i.d.) and demonstrates some benefits over 1.0 i.d. micro-bore column. The performance 2.1, columns were systematically compared. With column, loss apparent efficiency can be significantly reduced compared to in both isocratic gradient elution modes. In end, is almost comparable 2.1 from peak broadening point view. advantages vs narrow-bore are lower sample solvent consumption, frictional heating effects due decreased operating flow rates.